Secretion of Active Recombinant Human Tissue Plasminogen Activator Derivatives in Escherichia coli

• Main Authors: Manosroi J., Tayapiwatana C., Gotz F., Werner R.G., Manosroi A.
• 格式: Article
• 語言: English
• 出版: 2014
• 在線閱讀: http://www.scopus.com/inward/record.url?eid=2-s2.0-0035374839&partnerID=40&md5=b5d0751b280c50084061fb905fbd410a; http://www.ncbi.nlm.nih.gov/pubmed/11375177; http://cmuir.cmu.ac.th/handle/6653943832/4428

The DNA fragment coding for kringle 2 plus serine protease domains (K2S) of tissue plasminogen activator (tPA) was inserted into a phagemid vector, pComb3HSS. In the recombinant vector, pComb3H-K2S, the K2S gene was fused to gpIII of ΦM13 and linked to the OmpA signal sequence. The resulting gene, r...