A modified hybridoma technique for production of monoclonal antibodies having desired isotypes

• المؤلفون الرئيسيون: Napaporn Apiratmateekul, Ponrat Phunpae, Watchara Kasinrerk
• التنسيق: دورية
• منشور في: 2018
• الموضوعات: Biochemistry, Genetics and Molecular Biology; Chemical Engineering; Engineering
• الوصول للمادة أونلاين: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=71349083804&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/48867

In the present study, we describe a modified hybridoma technique for production of monoclonal antibodies (mAbs) having a desired isotype. Mice were immunized with the antigen of interest. After having reached a high antibody titer, cells expressing IgM or IgG molecules were isolated from spleen cell...