Cloning of D-glucose dehydrogenase with a narrow substrate specificity from Bacillus thuringiensis M15

• Main Authors: Boontim N., Yoshimune K., Lumyong S., Moriguchi M.
• 格式: Article
• 語言: English
• 出版: 2014
• 在線閱讀: http://www.scopus.com/inward/record.url?eid=2-s2.0-33750194390&partnerID=40&md5=54252ff523d00ec8fe549881df683507; http://cmuir.cmu.ac.th/handle/6653943832/5062

We have cloned an ORF of Bacillus thuringiensis M15, which encodes a protein sharing high similarity with D-glucose dehydrogenase. A high-expression plasmid (pBtGDH) for the ORF was constructed. Escherichia coli JM 109 transformed with pBtGDH exhibited D-glucose dehydrogenase activity, and the enzym...