Influence of Discontinuous PureSperm® and OptiPrepTM Gradient Centrifugations on Bovine Sperm Quality and the Sex Ratio of in vitro Produced Embryos

• Main Authors: Kakanang Buranaamnuay, Parisatcha Sangsuwan, Chinarat Changsangfa, Tassanee Faisaikarm, Kampon Kaeoket
• 語言: English
• 出版: Science Faculty of Chiang Mai University 2019
• 主題: dairy cattle; centrifugation; X-bearing sperm; in vitro fertilization; polymerase chain reaction
• 在線閱讀: http://it.science.cmu.ac.th/ejournal/dl.php?journal_id=5948; http://cmuir.cmu.ac.th/jspui/handle/6653943832/66139

Presently, separation of X- and Y-sperm with flow cytometry is only one successful method of sex selection; however, well-trained personnel and costly instrument are needed for this technique. Therefore, separation with a more simple and convenient method (i.e. a gradient centrifugation) becomes of...