Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059
A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and...
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2014
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th-cmuir.6653943832-75002014-08-30T04:11:40Z Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059 Sthitmatee N. Kataoka Y. Sawada T. A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens. 2014-08-30T04:11:40Z 2014-08-30T04:11:40Z 2011 Article 9167250 http://www.scopus.com/inward/record.url?eid=2-s2.0-82955251111&partnerID=40&md5=e41cb22858fcff790daa6023d043abba http://www.ncbi.nlm.nih.gov/pubmed/21747216 http://cmuir.cmu.ac.th/handle/6653943832/7500 English |
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Chiang Mai University |
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Chiang Mai University Library |
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Thailand |
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CMU Intellectual Repository |
| language |
English |
| description |
A mutant strain, PBA322, was constructed by electroporation of a phagemid containing the coding region of antisense RNA of the ompH gene, encoding 39 kDa capsular protein or OmpH, into the parental strain P-1059 (serovar A:3) of Pasteurella multocida, and the pathogenicity was determined in mice and chickens. Grayish colonies of the mutant, indicating loss of capsule synthesis, were observed under a stereomicroscope using obliquely transmitted light, while iridescent colonies were observed for the parental strain. Moreover, strain PBA322 showed a low amount of OmpH compared with the parental strain on SDS-PAGE. Additionally, the capsule of strain PBA322 was thinner than that of the parental strain according to electron microscopy, correlating to the attenuation against chickens. In conclusion, strain PBA322, the mutant of P. multocida strain P-1059, was completely attenuated for chickens. |
| format |
Article |
| author |
Sthitmatee N. Kataoka Y. Sawada T. |
| spellingShingle |
Sthitmatee N. Kataoka Y. Sawada T. Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059 |
| author_facet |
Sthitmatee N. Kataoka Y. Sawada T. |
| author_sort |
Sthitmatee N. |
| title |
Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059 |
| title_short |
Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059 |
| title_full |
Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059 |
| title_fullStr |
Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059 |
| title_full_unstemmed |
Inhibition of capsular protein synthesis of pasteurella multocida strain P-1059 |
| title_sort |
inhibition of capsular protein synthesis of pasteurella multocida strain p-1059 |
| publishDate |
2014 |
| url |
http://www.scopus.com/inward/record.url?eid=2-s2.0-82955251111&partnerID=40&md5=e41cb22858fcff790daa6023d043abba http://www.ncbi.nlm.nih.gov/pubmed/21747216 http://cmuir.cmu.ac.th/handle/6653943832/7500 |
| _version_ |
1681420811329601536 |