Roles of Arg427 and Arg472 in the binding and allosteric effects of acetyl CoA in pyruvate carboxylase

• Main Authors: Abdussalam Adina-Zada, Chutima Sereeruk, Sarawut Jitrapakdee, Tonya N. Zeczycki, Martin St. Maurice, W. Wallace Cleland, John C. Wallace, Paul V. Attwood
• Other Authors: University of Western Australia
• Format: Article
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/13592
• Institution: Mahidol University

Mutation of Arg427 and Arg472 in Rhizobium etli pyruvate carboxylase to serine or lysine greatly increased the activation constant (K a ) of acetyl CoA, with the increase being greater for the Arg472 mutants. These results indicate that while both these residues are involved in the binding of acetyl...