Evaluation of the Standard Diagnostics Leptospira IgM ELISA for diagnosis of acute leptospirosis in Lao PDR

• Main Authors: Ampai Tanganuchitcharnchai, Lee Smythe, Michael Dohnt, Rudy Hartskeerl, Manivanh Vongsouvath, Viengmone Davong, Olay Lattana, Paul N. Newton, Stuart D. Blacksell
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Immunology and Microbiology; Medicine
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/14278

The diagnostic utility of the Standard Diagnostics . Leptospira IgM ELISA for detection of acute leptospirosis was assessed in febrile adults admitted in Vientiane, Laos. Using the cut-off suggested by the manufacturer [optical density (OD) ≥0.75], the assay demonstrated limited diagnostic capacity with a sensitivity of 95% and a specificity of 41% compared with the . Leptospira microscopic agglutination test, which is the serological gold standard. However, re-evaluation of the diagnostic cut-off to an OD of 1.7 demonstrated improved diagnostic accuracy overall (sensitivity 70%; specificity 78%). © 2012 Royal Society of Tropical Medicine and Hygiene.