Construction of Bordetella pertussis strains with enhanced production of genetically-inactivated Pertussis Toxin and Pertactin by unmarked allelic exchange

• Main Authors: Wasin Buasri, Attawut Impoolsup, Chuenchit Boonchird, Anocha Luengchaichawange, Pannipa Prompiboon, Petre, Jean, Watanalai Panbangred
• Other Authors: Mahidol University. Faculty of Science. Department of Biotechnology
• Format: Article
• Language: English
• Published: 2017
• Subjects: Open Access article; Bordetella pertussis; Pertussis Toxin; Pertactin; allelic exchange
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/2726
• Institution: Mahidol University

Background: Acellular Pertussis vaccines against whooping cough caused by Bordetella pertussis present a muchimproved safety profile compared to the original vaccine of killed whole cells. The principal antigen of acellular Pertussis vaccine, Pertussis Toxin (PT), must be chemically inactivated to...