Characterization of key parameters for biotechnological lignocellulose conversion assessed by FT-NIR spectroscopy. Part I: Qualitative analysis of pretreated straw

• Main Authors: Chularat Krongtaew, Kurt Messner, Thomas Ters, Karin Fackler
• Other Authors: Mahidol University
• Format: Article
• Published: 2018
• Subjects: Chemical Engineering; Environmental Science
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/28882

Wheat straw (Triticum aestivum L.) and oat straw (Avena sativa L.) were subjected to acid and alkaline pre-treatments partly in combination with hydrogen peroxide. The aim was to remove lignin and increase the accessibility of the polysaccharides to enzymatic digestion. Accessibility was evaluated by digestion with a cell wall degrading enzyme complex to yield reducing sugars that may serve as precursor substrates for biofuels or building block chemicals. Changes in lignin, hemicelluloses, as well as amorphous, semi-crystalline, and crystalline regions of cellulose moieties of pretreated straw were efficiently characterized by Fourier transform near-infrared (FT-NIR) reflectance spectroscopy. These alterations of the chemical structure of straw after different pre-treatment methods were powerfully differentiated by principal component analysis (PCA). Characteristics of the different samples owing to the different pretreatment methods could be clustered from the PCA loadings spectra.