The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area
© 2014 The Society for Applied Microbiology. Aims: To evaluate the use of nonlocal, already-available strains of phages to indicate faecal contamination in Thailand waters. Methods and Results: Phages of Bacteroides fragilis strains ATCC 700786 (RYC2056PH) and ATCC 51477 (HSP40PH) were measured in 7...
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th-mahidol.334882018-11-09T09:26:59Z The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area K. Sirikanchana B. Wangkahad S. Mongkolsuk Chulabhorn Research Institute South Carolina Commission on Higher Education Chulabhorn Graduate Institute Mahidol University Biochemistry, Genetics and Molecular Biology Immunology and Microbiology © 2014 The Society for Applied Microbiology. Aims: To evaluate the use of nonlocal, already-available strains of phages to indicate faecal contamination in Thailand waters. Methods and Results: Phages of Bacteroides fragilis strains ATCC 700786 (RYC2056PH) and ATCC 51477 (HSP40PH) were measured in 71 human and animal wastewater samples in Thailand using a double-layer agar assay. Bacteriophage RYC2056PH was detected at concentrations comparable to representative human and animal wastewater samples from European and Mediterranean countries, with 61·7 and 33·3% above the threshold value of 100 PFU 100 ml<sup>-1</sup> in wastewater samples of human and animal origins, respectively. On the other hand, HSP40PH was detected at low concentrations in both human- and animal-polluted wastewaters. Moreover, RYC2056PH was found in 12 canal waters with human-influenced pollution and was not detected in 6 nonpolluted river waters being tested in this study. Conclusions: The presence of RYC2056PH could indicate nonsource-specific faecal contamination in Thailand. Significance and Impact of the Study: This study provided the first evidence that bacteriophages of the European-isolated B. fragilis strain RYC2056 could be used as nonsource-specific faecal indicators in the Southeast Asian region. The results of this study support the worldwide use of Bacteroides phages as faecal indicators. 2018-11-09T02:00:26Z 2018-11-09T02:00:26Z 2014-01-01 Article Journal of Applied Microbiology. Vol.117, No.6 (2014), 1820-1829 10.1111/jam.12646 13652672 13645072 2-s2.0-84920896809 https://repository.li.mahidol.ac.th/handle/123456789/33488 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84920896809&origin=inward |
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Biochemistry, Genetics and Molecular Biology Immunology and Microbiology K. Sirikanchana B. Wangkahad S. Mongkolsuk The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area |
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© 2014 The Society for Applied Microbiology. Aims: To evaluate the use of nonlocal, already-available strains of phages to indicate faecal contamination in Thailand waters. Methods and Results: Phages of Bacteroides fragilis strains ATCC 700786 (RYC2056PH) and ATCC 51477 (HSP40PH) were measured in 71 human and animal wastewater samples in Thailand using a double-layer agar assay. Bacteriophage RYC2056PH was detected at concentrations comparable to representative human and animal wastewater samples from European and Mediterranean countries, with 61·7 and 33·3% above the threshold value of 100 PFU 100 ml<sup>-1</sup> in wastewater samples of human and animal origins, respectively. On the other hand, HSP40PH was detected at low concentrations in both human- and animal-polluted wastewaters. Moreover, RYC2056PH was found in 12 canal waters with human-influenced pollution and was not detected in 6 nonpolluted river waters being tested in this study. Conclusions: The presence of RYC2056PH could indicate nonsource-specific faecal contamination in Thailand. Significance and Impact of the Study: This study provided the first evidence that bacteriophages of the European-isolated B. fragilis strain RYC2056 could be used as nonsource-specific faecal indicators in the Southeast Asian region. The results of this study support the worldwide use of Bacteroides phages as faecal indicators. |
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Chulabhorn Research Institute |
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Chulabhorn Research Institute K. Sirikanchana B. Wangkahad S. Mongkolsuk |
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Article |
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K. Sirikanchana B. Wangkahad S. Mongkolsuk |
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K. Sirikanchana |
title |
The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area |
title_short |
The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area |
title_full |
The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area |
title_fullStr |
The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area |
title_full_unstemmed |
The capability of non-native strains of Bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area |
title_sort |
capability of non-native strains of bacteroides bacteria to detect bacteriophages as faecal indicators in a tropical area |
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2018 |
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https://repository.li.mahidol.ac.th/handle/123456789/33488 |
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1763490375743307776 |