Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen
© 2018 Elsevier B.V. The quality of cat epididymal sperm cryopreserved and stored by four methods was assessed. Epididymal sperm were suspended in Tris-glucose-citrate egg yolk extender, loaded in 0.25 mL straws and then cryopreserved. The samples in a standard protocol (LN) were cryopreserved and s...
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th-mahidol.447732019-08-28T14:10:08Z Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen K. Buranaamnuay Mahidol University Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Veterinary © 2018 Elsevier B.V. The quality of cat epididymal sperm cryopreserved and stored by four methods was assessed. Epididymal sperm were suspended in Tris-glucose-citrate egg yolk extender, loaded in 0.25 mL straws and then cryopreserved. The samples in a standard protocol (LN) were cryopreserved and stored in liquid nitrogen (LN2). The sperm straws in the LN-Fr-LN group were cryopreserved in LN2 and stored in a −75 °C freezer; the straws were returned to LN2 prior to thawing. The loaded straws in the Fr group were transferred directly from 4 °C to the freezer and maintained in the freezer until thawing. The Fr-LN samples were cryopreserved and stored in the freezer and were introduced into LN2 before thawing. The sperm thawing was conducted on days 30, 60, 90 and 120 of cryopreservation. The sperm motility, viability, membrane integrity and acrosome integrity were evaluated at 15 and 180 min after thawing. The quality of post-thaw sperm in all three modified protocols was comparable (P > 0.05) and did not differ from that in the standard protocol except the membrane integrity of the 60 days stored samples evaluated at 15 min after thawing, which was significantly higher for the LN-Fr-LN than the Fr-LN groups (P = 0.04). The length of cryopreservation time had no effect (P > 0.05) on the sperm parameters assessed at 15 min after thawing. The sperm motility was significantly greater (P = 0.01 to P = 0.02) for the 15 min than the 180 min incubation. In conclusion, cat epididymal sperm could alternatively be cryopreserved and/or stored by using the −75 °C freezer for 120 days. To use, the cryopreserved sperm in the freezer could be thawed immediately or after being transferred to LN2. This was useful for the application of the −75 °C cryopreserved sperm in remote areas. 2019-08-23T10:17:52Z 2019-08-23T10:17:52Z 2018-04-01 Article Animal Reproduction Science. Vol.191, (2018), 56-63 10.1016/j.anireprosci.2018.02.008 03784320 2-s2.0-85042090716 https://repository.li.mahidol.ac.th/handle/123456789/44773 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85042090716&origin=inward |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Veterinary K. Buranaamnuay Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen |
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© 2018 Elsevier B.V. The quality of cat epididymal sperm cryopreserved and stored by four methods was assessed. Epididymal sperm were suspended in Tris-glucose-citrate egg yolk extender, loaded in 0.25 mL straws and then cryopreserved. The samples in a standard protocol (LN) were cryopreserved and stored in liquid nitrogen (LN2). The sperm straws in the LN-Fr-LN group were cryopreserved in LN2 and stored in a −75 °C freezer; the straws were returned to LN2 prior to thawing. The loaded straws in the Fr group were transferred directly from 4 °C to the freezer and maintained in the freezer until thawing. The Fr-LN samples were cryopreserved and stored in the freezer and were introduced into LN2 before thawing. The sperm thawing was conducted on days 30, 60, 90 and 120 of cryopreservation. The sperm motility, viability, membrane integrity and acrosome integrity were evaluated at 15 and 180 min after thawing. The quality of post-thaw sperm in all three modified protocols was comparable (P > 0.05) and did not differ from that in the standard protocol except the membrane integrity of the 60 days stored samples evaluated at 15 min after thawing, which was significantly higher for the LN-Fr-LN than the Fr-LN groups (P = 0.04). The length of cryopreservation time had no effect (P > 0.05) on the sperm parameters assessed at 15 min after thawing. The sperm motility was significantly greater (P = 0.01 to P = 0.02) for the 15 min than the 180 min incubation. In conclusion, cat epididymal sperm could alternatively be cryopreserved and/or stored by using the −75 °C freezer for 120 days. To use, the cryopreserved sperm in the freezer could be thawed immediately or after being transferred to LN2. This was useful for the application of the −75 °C cryopreserved sperm in remote areas. |
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Mahidol University |
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Mahidol University K. Buranaamnuay |
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K. Buranaamnuay |
title |
Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen |
title_short |
Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen |
title_full |
Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen |
title_fullStr |
Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen |
title_full_unstemmed |
Cryopreservation and storage of cat epididymal sperm using ‒75 °C freezer vs liquid nitrogen |
title_sort |
cryopreservation and storage of cat epididymal sperm using ‒75 °c freezer vs liquid nitrogen |
publishDate |
2019 |
url |
https://repository.li.mahidol.ac.th/handle/123456789/44773 |
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1763496603844345856 |