Improved yeast-screening assay for phytoestrogens
A yeast estrogen screening (YES) assay was improved to increase sensitivity for detection of phytoestrogens. New yeast strains minus one or the other of transporters Pdr5 or Snq2 and harboring yEGFP as a reporter gene were developed. The new strains showed 2-100-fold improvement in sensitivity for d...
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th-mahidol.761632022-08-04T15:49:59Z Improved yeast-screening assay for phytoestrogens Natthida Sophon Choladda Phiwpesh Sieng Darith Wichai Cherdshewasart Chuenchit Boonchird Chulalongkorn University Mahidol University Biochemistry, Genetics and Molecular Biology Chemistry Immunology and Microbiology A yeast estrogen screening (YES) assay was improved to increase sensitivity for detection of phytoestrogens. New yeast strains minus one or the other of transporters Pdr5 or Snq2 and harboring yEGFP as a reporter gene were developed. The new strains showed 2-100-fold improvement in sensitivity for detection of standard estrogens and antiestrogens. In addition, the assay time (1 h) using the newly developed strains was shorter than that (4 h) previously reported. Furthermore, the snq2-minus strains were most effective for detection of estrogenic activity while the pdr5-minus strains were most effective for detection of antiestrogenic activity. The efficacy of the new methods was evaluated and confirmed by testing with 23 Thai medicinal plant species. The new strains were also tested for detection of xenoestrogens. The results revealed that the newly developed YES methods were specific and rapid and suitable for simple high-throughput screening or detection of estrogen-like compounds. 2022-08-04T08:08:51Z 2022-08-04T08:08:51Z 2021-06-01 Article Bioscience, Biotechnology and Biochemistry. Vol.85, No.6 (2021), 1422-1432 10.1093/bbb/zbab051 13476947 09168451 2-s2.0-85107090923 https://repository.li.mahidol.ac.th/handle/123456789/76163 Mahidol University SCOPUS https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85107090923&origin=inward |
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Biochemistry, Genetics and Molecular Biology Chemistry Immunology and Microbiology Natthida Sophon Choladda Phiwpesh Sieng Darith Wichai Cherdshewasart Chuenchit Boonchird Improved yeast-screening assay for phytoestrogens |
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A yeast estrogen screening (YES) assay was improved to increase sensitivity for detection of phytoestrogens. New yeast strains minus one or the other of transporters Pdr5 or Snq2 and harboring yEGFP as a reporter gene were developed. The new strains showed 2-100-fold improvement in sensitivity for detection of standard estrogens and antiestrogens. In addition, the assay time (1 h) using the newly developed strains was shorter than that (4 h) previously reported. Furthermore, the snq2-minus strains were most effective for detection of estrogenic activity while the pdr5-minus strains were most effective for detection of antiestrogenic activity. The efficacy of the new methods was evaluated and confirmed by testing with 23 Thai medicinal plant species. The new strains were also tested for detection of xenoestrogens. The results revealed that the newly developed YES methods were specific and rapid and suitable for simple high-throughput screening or detection of estrogen-like compounds. |
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Chulalongkorn University |
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Chulalongkorn University Natthida Sophon Choladda Phiwpesh Sieng Darith Wichai Cherdshewasart Chuenchit Boonchird |
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Article |
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Natthida Sophon Choladda Phiwpesh Sieng Darith Wichai Cherdshewasart Chuenchit Boonchird |
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Natthida Sophon |
title |
Improved yeast-screening assay for phytoestrogens |
title_short |
Improved yeast-screening assay for phytoestrogens |
title_full |
Improved yeast-screening assay for phytoestrogens |
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Improved yeast-screening assay for phytoestrogens |
title_full_unstemmed |
Improved yeast-screening assay for phytoestrogens |
title_sort |
improved yeast-screening assay for phytoestrogens |
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2022 |
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https://repository.li.mahidol.ac.th/handle/123456789/76163 |
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