สภาวะสำหรับเพิ่มจำนวนเซลล์ต้นกำเนิดเม็ดเลือดของเลือดจากสายสะดือรกของเด็กแรกเกิด

• Main Author: นิดา พรประเสริฐสุด
• Other Authors: นวพรรณ จารุรักษ์
• Format: Theses and Dissertations
• Language: Thai
• Published: จุฬาลงกรณ์มหาวิทยาลัย 2005
• Subjects: เลือดทารกในครรภ์; สายสะดือ; สเต็มเซลล์; Fetal blood; Umbilical cord; Stem cells
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:30321
• Institution: Chulalongkorn University

Allogeneic transplantation with umbilical cord blood (UCB) is limited in adult recipients by a low stem cell dose. The ex vivo expansion of cord blood (CB) hematopoietic stem and progenitor cells (HSPCs) is the current strategy to overcome this problem. Many investigators have used xenogeneic, especially murine, stromal cells and fetal calf serum. Because of the possible transmission of infectious diseases, we using primary human mesenchymal stem cells (MSCs) to established a novel serum-free culture system to expand human CB HSPCs. CB CD133" -enriched cells were culture for 28 days in serum-free medium supplement with interleukin (IL)-la or with flt3/flk2 ligand (FL) and thrombopoietin (TPO) in presence or absence of MSCs and analyzed for proliferation, phenotype, and clonogenic potential. Significant expansion of CB nucleated cells were achieved in the presence of human MSCs, while CB HSPCs were achieved in MSCs treated with FL and TPO condition. The differentiative potential of CB CD133+-enriched cells cocultured with human MSCs was primirily shifted toward the myeloid lineage, while maintaining/expanding lymphoid population. Clonogenic analysis of the expanded cells showed increases in colony-forming unit-granulocyte, macrophage (CFU-GM) and colony-forming unit- granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM); These results indicate that adult human MSCs in the presence of approprite cytokines can be used to efficienty expand/maintatin myeloid and lymphoid cell populations from human CB HSPCs.