MORFOLOGI PERKECAMBAHAN BIJI ROTAN GETAH (DAEMONNROPS MELANOCHAETE BLUME
The Morphology of Germination of Rotan get-eh Seed (Daemonorops melanochaete Blume) A morphological study of seed germination of rotan geteh (Daemonorops melanochaete, Blume) was carried out to obtain information concerning the developmental changes of the process. Seeds were divested of the sarcote...
Saved in:
| Main Author: | |
|---|---|
| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/1017 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The Morphology of Germination of Rotan get-eh Seed (Daemonorops melanochaete Blume) A morphological study of seed germination of rotan geteh (Daemonorops melanochaete, Blume) was carried out to obtain information concerning the developmental changes of the process. Seeds were divested of the sarcotesta, imbibed in distilled water for 12 hours and placed on filter paper in pete dishes to germinate. Germination occured within 10 days. Seedlings at their 2nd leaf stage were then transferred to garden soil mixed with sand in plastic bags and growth was followed instil the emergences of the 3rd leaf. Each seed showed the development of 1 to 7 embryos, but up till 4 seedlings only grew further from one seed. For the anatomical study, parts of the embryo at. various stages of development were killed and fixed in CRAF III or FAA, dehydrated with the tertiary butyl alcohol series and then infiltrated and embedded in paraffine. Serial section at 8 - 10 /am were cut with a rotary microtome and stained with haematoxylin. The embryo consists of a curved axis with the plumula and radicle at its respective ends, surrounded by a single cotyledon. Germination commences when cell proliferation occurs in the proximal part of the cotyledon resulting in an outgrowth that pushes and tears part of the seed coat outwards, and a whithish coloured button-like structure then appears at the seed coat surface. Cell division followed by cell elongation in the area lateral and distal to the plumula-radicle axis, pushes this part o: the embryo outwards also. The plumula then grows in height.Adventive roots appear at the base of the stem followed by emergence of the 2nd leaf. The distal part of the cotyledon remains in the seed and develops into a haustoriurn. This development involves division and growth of parenchyma cells and formation of intercellular spaces while the endosperm decreases and finished when the 2nd leaf fully expanded. The vascular bundles of the cotyledon mature at the time of active growth of the plumula and radicle. In an ungerminated seed, protein can be shown but starch is present only after germination |
|---|