ENHANCEMENT OF ARTEMISININ PRODUCTION IN CELL SUSPENSION CULTURE OF ARTEMISIA ANNUA L. WITH ELICITATION BY YEAST EXTRACT

• Main Author: SINTHARI RAHAYU (NIM 10704128), DIAN
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/10235
• Institution: Institut Teknologi Bandung

Artemisinin, a sesquiterpene lacton, is a secondary metabolite of Artemisia annua L. Since a need for artemisinin is high, method that can enhance the artemisinin are needed. One of them is cell cultures. Yeast extract (Saccharomyces cereviseae Hansen.) can be used as an elicitor to increase secondary metabolite in cell suspension culture. The aim of this study is to enhance the production of artemisinin from cell suspension culture of Artemisa annua L. with elicitation. Callus initiation was done using Murashige & Skoog (MS) medium supplemented with growth hormone NAA (2 mg/L) + BAP (0,5 mg/L ) for Artemisia annua L. Elicitation process was done by adding elicitors yeast extract 0.01, 0.05 and 0.1 to MS liquid medium. Isolation of artemisinin was done by maceration method using methanol and liquid-liquid extraction method using n-hexane. The extracts were cheeked by thin layer chromatography (TLC) and the concentration was calculated by spectrophotodensitometri. Concentration of artemisinin can be increased up to 2.38 times in cell suspension culture of Artemisa annua L. with yeast extract 0.1% (b/v) at 14th day. Yeast extract elicitors could enhance the concentration of artemisinin in cell suspension culture Artemisia annua L.