TRANSFER DNA BACILLUS SUBTILIS KE DALAM SEL SACCHAROMYCES CEREVISIAE

• Main Author: Sudding
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/1071
• Institution: Institut Teknologi Bandung

This study concerns about the feasibility of transformation into an eucaryotic system, as applied to Saccharomyces cerevisiae as the recipient and Bacillus subtitis for DNA donor. The primary purpose is to determine suitable conditions to undertake the transformation. <br /> The process of transformation begins by isolating the DNA of Bacillus subtilis by using-lysozyme to break the cell wall. The DNA is afterwards extracted from the mixture of other cell components by using organic solvents, then coagulated with ethanol 90 %. The formation of Saccharomyces cerevisiae's protoplasm requires the cellulase enzyme extracted from the hepatopancreas of the edible snail (Achatina fulica) as well as lysozyme, which is done in an osmotically stabilized buffer solution. The transformation is also feasible in a solution of PEG into which CaC12 is added to induce the introduction of DNA into the cell. <br /> The transformated cells grown both on YDPA and NBA medium were able to hydrolize starch and gelatine, in addition it was able to convert starches and glucose into alcohol. The recombinant cells also produce an antibiotic substance able to inhibit the growth of fungi. The morphology of the recombinant cells showed a likeness to that of both Saccharomyces cerevisiae and Bacillus It can be concluded that cell transformation by means of protoplasm fusion is applicable between eucaryotic and procaryotic systems. It is hoped that this research might be continued as to study other properties that has not been studied yet.