#TITLE_ALTERNATIVE#

• Main Author: FITRIANI (NIM 10704065), LILI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/10804
• Institution: Institut Teknologi Bandung

Poly (D,L-lactide acid) has been used as scaffold and controlled release device for protein such as growth factor in tissue engineering. In this study, PDLLA microparticles were made in two types, porous and non-porous microparticles and papain was used as model protein. Porous microparticles were proposed to reduce burst release of protein and to prevent diffusion of released protein into non-target tissue, whilst non-porous microparticles were used as delivery system of the protein. Formulation of porous microparticles was made by water-oil-water (W1/O/W2) emulsification-solvent evaporation using gas foamed as porogen. Variations of the amount of sodium bicarbonate, volume of citric acid solution and time for homogenization were optimized to produce optimum formulation. Evaluation for this microparticles included morphology of particles, particle size distribution and porosity. Protein was encapsulated in microparticles using W1/O/W2 and solid-oil-water (S/O/W) emulsification-solvent evaporation. Types of encapsulation methods and ratios of papain-PEG 20000 were observed in this study to provide the highest encapsulation efficiency. Porous microparticle produced by ratio volume of acid : dichloromethane : poly(vynil alcohol) (PVA) = 1:3:3 and the ratio of sodium bicarbonate : PDLLA = 2:3 was the optimum formulation. Hydrogel could not be encapsulated within microparticles, therefore will be added into prepared microparticles. The entrapment efficiency made by W1/O/W2 method was 6,38%+-0,025, whilst S/O/W using ratios of papain-PEG 20000 1:1 ; 1:4 ; and 1:5 were 6,24%+-0,91 ; 30,15%+-1,66 and 60,67%+-4,93, respectively. To conclude, porous microparticle can be produced using gas foamed as porogen and S/O/W is the best method to encapsulate protein with highest entrapment efficiency.