PENERAPAN METODE ISOLASI ALKALOID MELALUI PEMBENTUKAN KOMPLEKS BISMUT DALAM ISOLASI ALKALOID DARI BIJI JINTAN HITAM (NIGELLA SATIVA L.)
Extraction and chromatographic methods are commonly applied in isolating alkaloid compounds from natural sources. In this research, a new isolation method of alkaloid was developed using caffeine and papaverine HCl as a model. Prior to alkaloid isolation, a selective reaction was performed to precip...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/10827 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Extraction and chromatographic methods are commonly applied in isolating alkaloid compounds from natural sources. In this research, a new isolation method of alkaloid was developed using caffeine and papaverine HCl as a model. Prior to alkaloid isolation, a selective reaction was performed to precipitate the alkaloids using salt solution of bismuth. The cation was then dissociated from the precipitate by adding sodium-ethylendiamintetraacetate. The method was optimized by finding molar ratio of the cation and finding an appropriate pH. It was found that each of 1 mole caffeine and 1 mole papaverine HCl required 6 moles and 9 moles of cationic bismuth respectively and an appropriate pH of the reaction is 2. The effectiveness of alkaloid isolation by this method was more than 90%. This method applied to isolate alkaloid from Nigella sativa L. seeds gave a yield of 0.13%. |
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