PENGARUH ALLOPURINOL TERHADAP PERKEMBANGAN PRALAHIR MENCIT (MUS MUSCULUS ) SWISS WEBSTER

• Main Author: A. Basuki, Yuniarti
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/1084
• Institution: Institut Teknologi Bandung

Effects at allopurinol Q. prenatal development at swiss Webster atbin_o dice I Mus_ musiij1us A. This study was conducted to investigate the effects of allopurinol on prenatal development of albino Swiss Webster mice (\"thus musculus ). Allopurinol suspended in 0.5 % C.M.0 was administered as a single dose intraperitoneally into pregnant mice with dosages of 50, 75 and 100 mg/kg body weight on day 8 or 10 of gestation. The results revealed intrauterine death, external malformations, cleft palate and skeletal malformations. The incidence of intrauterine death on day 8 of gestation was very significantly different (p<0.01) from the control in the dose 75 mg/kg b.w and significant (p<0.05) in the dose 100 mg/kg b.w. Litter size of mice treated on day 8 of gestation was very significantly decreased (p<0.01) from the control in the dose 75 mg/kg b.w. and significant (p<0.05) in the two doses 50 and 100 mg/kg b.w. Body weight of live fetuses was very significantly decreased (p<0.05) from the control in the two doses 50 and 100 mg/kg b.w. when the mice were treated on day 8 or 10 of gestation. The malformations obtained were exencephaly, open eyelid, kinky tail and cleft palate. Exencephaly and cleft palate were the only significant malformations occured . Skeletal malformations obtained were fused ribs and delayed ossification of supraoccipital bone, cervical vertebral body, sternebrae and tarsal bone. The incidence of delayed ossification of cervical vertebrae body was very significantly different from the control (p<0.01) in thetwo doses 75 and 100 mg/kg b.w., and delayed ossification of supraoccipital bone, sternebrae and tarsal bone were significant (p<0.05) only in mice treated on day 8 of gestation. Skeletal variations obtained were supernumarary ribs, 14th unilateral rib, 14th short ribs and dumbbell sternebrae. It could be concluded, that allopurinol is embryotoxic, teratogenic and delay ossification processes.