#TITLE_ALTERNATIVE#

• Main Author: YONI SANTIKA (NIM 10704064), PRATIWI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/11151
• Institution: Institut Teknologi Bandung

Bone graft is currently the primary therapy method for bone healing, but this method still has various disadvantages. Tissue engineering provides a promising alternative to replace bone graft by inducing bone formation naturally using bone growth factors. Bone morphogenetic protein-2 (BMP-2) is a member of growth factors with strong osteoinductive activity. This research was intended to synthesize the coding region of synthetic BMP-2 using Thermodynamically Balanced Inside-out (TBIO) bidirectional synthesis method based on two step Polymerase Chain Reaction (PCR) and its cloning into pGEM-T cloning vector in Escherichia coli JM109. Double cleavage analysis using BamHI and XhoI restriction enzyme and PCR analysis showed one band with 355 base pairs (bps) in size and sequencing analysis of insert DNA in recombinant plasmid showed that insert DNA in K and M transforman plasmids have 100% similarity with the nucleotides sequence of coding region of synthetic BMP-2. This result proved that coding region of synthetic BMP-2 with no mutation was inserted into pGEM-T plasmid.