#TITLE_ALTERNATIVE#

• Main Author: SELLA AUGUSTA BULAN (NIM 10704009), R.
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/11340
• Institution: Institut Teknologi Bandung

Centella asiatica (L.) Urban contains asiaticoside substance which belong to triterpenoid group. Tritepenoids of Centella asiatica (L.) Urban were known to have pharmacological effects, such as healing duodenal ulcer, wound healing, etc. Tissue culture methods were used to increase multiplicate pegagan tissue. This reasearch was aimed to investigate the influence of methyl jasmonate and copper ion on the production of asiaticoside in pegagan tissue culture. Callus initiation were done using Murashige-Skoog solid medium supplemented with growth hormone NAA (1 mg/L) and BAP (1 mg/L). Callus were subcultured to liquid medium that produced cell suspension culture. Elicitors were methyl jasmonate with concentration 50, 100 dan 200 uM and copper with concentration 10 dan 25 uM on liquid medium. Samples were extracted with maceration method. Asiaticoside were determined by spectrodensitometry method. Chromatogram showed that the highest content of asiaticoside was found from cell suspension culture elicitated with methyl jasmonate at 100 uM on day 14 compared to pegagan leaves (1,71 fold), control suspension (1,25 fold) and 8 weeks callus (4,94 fold) and elicitation with copper 25 uM on day 21 compared to pegagan leaves (1,44 fold), control suspension (6,76 fold) and 8 weeks callus (4,16 fold). <br />