#TITLE_ALTERNATIVE#

• Main Author: NURHAYATI (NIM 10704034), SRI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/11434
• Institution: Institut Teknologi Bandung

Chitinase is an enzyme that hydrolyzes B-1,4 link between N-acetyl-D-glucosamine in chitin resulting N-acetyl-D-glucosamine monomers. Chitinase plays role in chitin cleavage to produce glucosamine which is subsequently used as bone supplement. This research screened chitinase activity from bacteria isolated in swamp, shrimp pond, and basin. Screening of chitinase activity was done using chitin-containing bacterial medium. Chitinase activity was determined by clear zone caused by chitin degradation. The bacterial chromosomes were isolated. The 16S rDNA gene were amplified using Polymerase Chain Reaction (PCR) The PCR products were sequenced by Sanger method. The species identity of bacteria was determined by aligning the 16S rDNA sequence gene in Basic Local Aligment Search Tool (BLAST) from National Centre of Biotechnological Information (NCBI) program. There are two bacterial isolates which have the highest chitinase activity. One of the isolates was identified as Aeromonas hydrophila strain BJ and the other one was identified just as Aeromonas sp.; the exact species has not been able to be identified yet.