#TITLE_ALTERNATIVE#

• Main Author: TRUSMIYADI (NIM 13003029), YADI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/11627
• Institution: Institut Teknologi Bandung

Bioethanol is a derivate product from cassava that is thoroughly developed nowadays. Bioethanol pilot plant in Laboratorium Pilot Program Studi Teknik Kimia Institut Teknologi Bandung has applied the procedure that had been tested in laboratory scale in 2005. Pilot plant ability to produce ethanol has been proven in broth fermentation capacity 10 liters per day with ethanol concentration 8-9%-v. One program of bioethanol roadmap research in 2006 is to increase productivity and workrate of &#945;-amylase dan glukoamylase Aspergillus niger for saccharification in bioethanol production.<p> <br /> <br /> <br /> The objective of this research is to determine optimum condition of starch saccharification. The workrate of amylase enzyme extract is analyzed with iodine to determine the workrate of &#945;-amylase and Somogyi-Nelson to determine the workrate oh glukoamylase. Researched variable to determine the optimum performance condition of amilase extract enzyme are acidity (pH 3,5-8), temperature (25-80 degrees C), Ca2+ concentration (25-200 ppm), substrat concentration (0,5-20 %-b/v), and enzyme percentage (5-50%-v).<p> <br /> <br /> <br /> The results of this research show that the optimum condition for amylase are pH 4,5, temperature 60 degrees C, Ca2+ concentration 75 ppm, and substrat concentration 7 %-b/v. The workrate of amylase enzyme extract is increasing with the increasing in amount of enzyme percentage, but the increasing is limited by glucose concentration in enzyme because high glucose concentration enforce product inhibiton of enzyme.