Optimation Fatty Acid Production of Marine Microalgae Thalassiosira sp.

• Main Author: EWIDYASARI ANWARD (NIM 10505037), ETSUROYYA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/12083
• Institution: Institut Teknologi Bandung

Microalgae show a potential raw material for the use of alternative energy because they have high lipid content. The purpose of this research is to optimize of fatty acid production from marine microalgae Thalassiosira sp. Batch culture was used to cultivate microalgae using bubbling air or aeration with CO2. Microalgae cells obtained was then pretreated by the use of sonication or freeze drying, before their lipid content were extracted with organic solvents of n-hexane or chloroform:methanol (1:1). Total fatty acid content was determined by the method of Gravimetri, and the fatty acid characteristic was analyzed using the Gas Chromatography-Mass Spectrometry (GC-MS) method. The microalgae biomass concentration obtained from the culture using bubbling air or aeration with CO2 was 0.4903 and 0.3052 g/L, respectively. The total fatty acid content obtained from cells cultivated using bubbling air or aeration with CO2; cells pretreated by sonication or freeze drying; and cells extracted by solvents of n-hexane or chloroform:methanol (1:1) was 51.4 or 41.2%; 51.4 or 39.4%; and 37.3 and 39.4%, respectively. These result suggested that the maximum fatty acid production when microalgae cells was bubbling with air and then pretreated by sonication prior to extract their lipid using chloroform:methanol (1:1) as solvent.