Mitochondrial DNA Nucleotide Mutations of a 120 Years Old Indonesian Man

• Main Author: SRI NURHASANAH (NIM 10504020), EUIS
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/12085
• Institution: Institut Teknologi Bandung

Mitochondria have their own DNA which is maternally inherited and is highly variable, particularly at the hypervariable I (HVSI) and hypervariable II (HVSII) segments of the control region D-Loop. Accumulation of mitochondrial DNA (mtDNA) mutations may cause mitochondrial failure, which can further lead to the tissue dysfunction. Accumulation of mtDNA mutations is an important contributor to the ageing process, resulting in the major risk factor of degenerative diseases. There are huge numbers of researches dealing with ageing-related mtDNA mutations have been done, but none of mtDNA mutations data from Indonesia has been reported. In this research, mtDNA mutations of Indonesian people aged 120, 35, and 21 years-old were analyzed. mtDNA templates from mouth epithelial cells were obtained by lysis and then amplified by PCR. Visualization of PCR results by agarose gel electrophoresis showed one band of 0,9 kb DNA. Nucleotide sequence of the PCR results was determined by the Dideoksi Sanger method and was compared to CRS (Cambridge Reference Sequence). The result showed that mutation at position 16032-16194 in an old person is more frequent than in younger ones. It is suggested that further analysis on the whole mitochondrial genome and mtDNA samples from other aged people is important to confirm whether this research result is related to the ageing process. <br /> <br />