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Mitochondria have their own genetic material which is inherited from the maternal. Mutation that occurred in mitochondria DNA can cause genetic disease. One of the diseases associated with DNA mutation mitochondria is diabetes mellitus which accompanied by deafness (Maternally Inherited Diabetes and...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/12123 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Mitochondria have their own genetic material which is inherited from the maternal. Mutation that occurred in mitochondria DNA can cause genetic disease. One of the diseases associated with DNA mutation mitochondria is diabetes mellitus which accompanied by deafness (Maternally Inherited Diabetes and Deafness; MIDD), namely the A3243G mutation. Based on the results of earlier research, DNA mutan to this case is shared with normal DNA, known as heteroplasmy. A sample of MIDD shows PCR alleles specific alignment (PASA)-positive on the A3243G mutation. However, direct sequencing through the mutation is not identified. Research was conducted to determine nucleotide sequence by cloning method. Research conducted includes PASA, construction recombinant DNA, cloning, screening, and sequencing. Visualization of PCR results show DNA fragment size of 190 pb. In the cloning phase obtained 28 white colonies. Based on sequencing result, nucleotide sequence of two colonies are same with Cambrige Reference Sequence (CRS). Eventually, can conclude that the two colonies is derived from normal mtDNA. <br />
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