Cloning Gene Encoding Glucoamylase Saccharomycopsis fibuligera R64 in pGEM-T Vector
Glucoamylses are produced by various organisms, such as fungal Aspergillus awamori, yeast Saccharomycopsis fibuligera, and bacterium Thermoanaerobacterium thermosaccharolyticum. Glucoamylase has a potential prospect to be developed and applied in industries, such as glucose syrup, bread, and bioetha...
Saved in:
| Main Author: | |
|---|---|
| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/12499 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Glucoamylses are produced by various organisms, such as fungal Aspergillus awamori, yeast Saccharomycopsis fibuligera, and bacterium Thermoanaerobacterium thermosaccharolyticum. Glucoamylase has a potential prospect to be developed and applied in industries, such as glucose syrup, bread, and bioethanol industries. Wild type microorganism-producing glucoamylase normaly express low level of enzyme, while high level production and low cost enzymes are needed in many industries. To obtain strains with superior properties, a recombinant DNA is used and Pichia pastoris is a suitable expression host for producing glucoamylase at high level. The aim of this research was to amplify a gene encoding glucoamylase S. fibuligera R64 (GLL) and to clone the PCR product in pGEM-T vector. |
|---|