Biodegradation of Monochloroacetic Acid by Pseudomonas pseudoalcaligenes

• Main Author: APRIANI (NIM 10505035), ULI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/12744
• Institution: Institut Teknologi Bandung

Monochloroacetic acid (MCA) is a chlorinated aliphatic hydrocarbon that commonly used as herbicides. MCA is produced at around 300,000 tonnes annually. MCA is a strong acid, highly soluble in water and not likely volatilize out of water, so it commonly persistence in the environment and have toxic effects to human, animals, plants, and algae. Degradation of this compound into non hazardous metabolites could reduce its toxicity. In this study, MCA is degraded by Pseudomonas pseudoalcaligenes. This microorganism could produces dehalogenases. 2-Haloacid dehalogenases catalyzes the dehalogenation of 2-haloalkanoic acids to produce a corresponding 2-hydroxylkanoic acids. Pseudomonas pseudoalcaligenes was grown in Luria-Bertani (LB) medium containing 400-900 ppm of MCA. Cells were incubated at 30oC on a rotary shaker at 200 rpm for 12 hours. The results showed that Pseudomonas pseudoalcaligenes could not growth well in MCA containing LB medium higher than 500 ppm. The biodegradation of 500 ppm MCA could occured 100% on pH 7 and 30 oC with incubation time of 20-24 hours.