LIPASE FROM THERMOPHILES AS A CATALYST OF TRANSESTERIFICATION REACTION IN BIODISEL SYNTHESIS

• Main Author: FITRI (NIM : 10508078); Pembimbing : Fida Madayanti Warganegara, Ph.D, AMALIA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/14829
• Institution: Institut Teknologi Bandung

Biodiesel synthesis was achieved through the transesterfication reaction between triglyceride <br /> <br /> <br /> <br /> <br /> and alcohol. This reaction can be catalyzed by lipase enzyme, but thermozyme was needed since the reaction occured in a quite high temperature. Bacilus sonorensis sp, isolated from the thermophile phase in the composting phase, can be a source of thermozyme. This research begins with the rejuvenation of the B. sonorensis culture and the production of lipase enzyme. The crude enzyme extract was fractinated using aceton in 3 step : 0-30%, 30-50% and 50-70%. Assay highest lipase specific activity showed in fraction 30-50%, which is 92.8 U/mg protein. This fraction was characterized by its optimum pH (7 and 9) and optimum temperature (60oC). The Native-PAGE and zymogram results showed that there's 2 protein's tape that has lipase <br /> <br /> <br /> <br /> <br /> activity. Transesterification reaction was done using triolein and methanol. . Methyl oleic's peak was found in the biodiesel characterization with ESI-MS which showed that lipase from B.sonorensis has a transesterification activity. This enzyme is expected has a regioselectivity on <br /> <br /> <br /> <br /> <br /> the ester bonds on carbon 1,3 or 2,3 position from triglyceride and efficiency number around 83% with 0.56 (mu)g/mL substrate converted to product in every hour.