#TITLE_ALTERNATIVE#

• Main Author: NOOR SALLAM (NIM : 10706011); Pembimbing : Dr. As’ari Nawawi; Dr. Saleh Wikarsa, ANDI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/14873
• Institution: Institut Teknologi Bandung

Spinach is a plant commonly used by Indonesian as vegetable and has been used as traditional medicine for kidney disorders and dysentery that also contains chlorophyll which has also some pharmacological effects, such as of antioxidant and anticancer. This research is aimed to make a stable chlorophyll derivate, known as chlorophyllin from spinach leaves and to develop formulations a lozenges containing chlorophyllin. Spinach was macerated in 80% acetone in order to extract the chlorophyll. Chlorophyll extract was then derivated by adding CuCl2 to make a heat-resistant chlorophyll derivate. Cu-pheophytin fraction as a heat resistant derivate was then derivated by adding 0,5M NaOH to make a heat-resistant and water soluble chlorophyll derivate. Lozenges formulation was developed by using wet granulation method by varying types of binder and filler composition which is compatible to the formula. Lozenges obtained were characterized involving uniformity of size and weight, hardness, frictibility, friability, and the amount of chlorophyll in tablets which was determined using UV-visible spectrophotometry. Chlorophyllin was heat resistant and soluble in water. Chlorophyllin lozenges was formed from the best placebo lozenges performance containing 5% gelatin and PEG 6000 as the binder and the filler used were sucrose and mannitol (4:1). Lozenges weight varied between 1,91 to 2,18 grams, diameter of 2,01 to 2,05 cm, thickness of 0,57 to 0,60 cm, hardness of 8,5 to 10,5 kg/cm2, and the friability and frictibility less than 1%. By chemical determination, the chlorophyllin was still intact or identified in the lozenges. Chlorophyllin can be formed from the derivatitation of the chlorophyll extract and can be formulated into lozenges.