#TITLE_ALTERNATIVE#

• Main Author: HARIYADI KURNIANTO (NIM : 23709008); Tim Pembimbing : Dr. Ir. Hermawan Judawisastra; Dr. Ir. Bamb
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/15859
• Institution: Institut Teknologi Bandung

Limitation to the number of donors for transplantation is a serious problem, which need for an alternative replacement material that can overcome this limitation. In this study, alginate-chitosan scafffold has been succesfully synthesized by freeze drying method. To improve the performance of scaffold, it is needed a material as initiator for new cell growth. In Tissue Engineering, the materials for initiator of new cell growth are required to have good properties of biocompatible, bioactive and biodegradable. To meet the requirment of those properties, it needs to modificate alginate-chitosan membrane by growing hydroxyapatite (HAp) on the membrane surface. Alginate and chitosan have opposite ion charge, where the chitosan has a positive charge and the alginate has a negative charge. The mixing of chitosan and alginate will form two stable chemical bonds that increase their structural integrity. Mixture of chitosan and alginate can form a scaffold membrane by freeze drying process in relatively short time and easy to set the level of porosity. Alginate-chitosan membrane support was use as growing media for HAp with Simulated Body Fluid (SBF) solution was used as a source of HAp. The growth oh <br /> <br /> <br /> <br /> <br /> HAp was synthesized by self assembly method with immersing time was varied by 1 day and 4 days. Scanning Electron Microscope (SEM) characterization of freeze drying process of membrane support showed porous morphology with average diameter of 95&#956;m, which closed to comercial product. X-ray Diffraction (XRD) characterization showed the formation of HAp phase with index reference at 2&#952; of around 32o - 33o, where degree of crystalinity of HAp was increase along with the immersion time.