MODIFICATION OF METAL ION CENTER FROM NATURAL CHLOROPHYLL : STUDY POTENCY AS PHOTOSENSITIZER PHOTODYNAMIC INACTIVATION

• Main Author: OKTAVIA (NIM : 10508006); Pembimbing 1 : Dr.Irma Mulyani; Pembimbing 2 : Dr. Veinardi Sue, LISTIANA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/16294
• Institution: Institut Teknologi Bandung

Chlorophyll is one of high abundance pigment in nature. Chlorophyll has a unique structure which is a porphyrin ring coordinated to ion Mg2+. The chlorophyll has a many conjugated electron, therefore the chlorophyll is a sensitive compound from visible light. This characteristic gives chlorophyll a potency as a photosensitizer especially in PDI (Photodynamic Inactivation). In this research, the effect of metal central ion that coordinated to chlorophyll was studied on the inhibition of bacterial growth using Staphilococcus aureus. The chlorophyll a and b were isolated from green spinach using the dioxane-water mixture. Both the chlorophyll were separated using the sucrose column chromatography technique. The separation products were charaterized by TLC, and absorption spectrophotometry. The TLC results showed that the Rf of chlorophyll a was higher than chlorophyll b. On the <br /> <br /> <br /> <br /> <br /> <br /> <br /> absoption spectra, the maxima of chlorophyll a observed at 411 and 663 nm, where the of chlorophyll b were at 423 and 659 nm. The central ion Mg2+ of chlorophyll a was replaced <br /> <br /> <br /> <br /> <br /> <br /> <br /> by ion Cu2+ or Zn2+ and the products were characterized by emission spectrophotometry and tested their bioactivity on Staphilococcus aureus. The emission spectra showed that intensity chlorophyll a> Zn-pheophytin a> Cu-pheophytin a. Bioactivity of Chlorophyll a, Cu-Pheophytin a and Zn-Pheophytin a respectively was tested on Staphilococcus aureus growth. The mixtures wereradiated under red light (&#955;= 650 nm) for 30 minutes, before incubated for 24 hours. The results show that the Zn-Pheophytin a ( 66.8%) gives higher inhibition of than chlorophyll a (30.1 %). Whereas, the Cu-Pheophytin a was unable to inhibit the bacterial <br /> <br /> <br /> <br /> <br /> <br /> <br /> growth, it was propably due to the lack of singlet oksigen formation. The inhibition of bacterial growth without radiation was smaller than the presence of radiation.