EXTRACTION AND CHARACTERIZATION BIOPIGMENT FROM MARINE MICROALGAE NANNOCHLOROPSIS SP.

• Main Author: IRVA SARI ( NIM : 10508067); Pembimbing I : Dr. Zeily Nurachman Pembimbing II : Dr. Santi Nur, MILA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/16452
• Institution: Institut Teknologi Bandung

Biopigments that are typically obtained from vegetables and fruits are used for many applications such as foods, cosmetics, pharmaceuticals, dyes, energy, and medicine. <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> Microalgae are a potential organism for producing biopigment because they have several advantages compared with other crops, such as can be cultivated easily, does not require a large area, and grow quickly. The objective of the present study is to identify biopigment from biomass of marine microalgae Nannochloropsis sp. To achieve this objective, research steps include cultivating microalgae in two different media:Walne and modification, harvesting wet biomass, extracting biopigments, separating biopigments, and identifying microalgal biopigments. The cell density obtained from the culture in Walne and modification medium for 6 d was 31.78 x 106 and 17.73 x 106 cells/mL, respectively, while, the concentration of wet biomass obtained from the culture for 14 d was 4.76 and 3.09 g/L, <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> respectively. Nannochloropsis sp. pigments obtained from the culture both in Walne and modification medium contained beta-carotene, pheophytin a, chlorophyll c, chlorophyll a, <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> xantophyll, and two unknown biopigments. Rf value (percentage) of biopigments obtained from the culture Walne medium was beta-carotene 0.27 (22.10%), pheophytin a 0.41 (16.35%), chlorophyll c 0.56 (30.44%), chlorophyll a 0.62 (4.17%), xantophyll 0.83 (3.30%), unknown biopigment I 0.59 (19.64%), and unknown biopigment II 0.96 (3.99%). Meanwhile, Rf value (percentage) of biopigment obtained from modification medium was beta-carotene 0.19 (6.66%), pheophytin a 0.41 (9.10%), chlorophyll c 0.55 (20.43%), chlorophyll a 0.59 (23.35%), xantophyll 0.80 (8.23%), unknown biopigment I 0.57 (1.98%), and unknown biopigment II 0.97 (18.25%). The result suggested that Nannochloropsis sp. was potential organisme for biopigment production. <br />