ISOLATION AND CHARACTERIZATION OF BIOPIGMENT FROM MICROALGA THALASSIOSIRA SP.

• Main Author: ROMAYANTI (NIM : 10508093); Pembimbing 1 : Dr, Santi Nurbaiti; Pembimbing 2 : Zeily Nurachman, SUCI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/17314
• Institution: Institut Teknologi Bandung

Microalgae is a natural resource which is very abundant. The study of microalgae as a source of biomass to produce biodiesel has been widely applied. However, research biopigment microalgae as a source of highly benficial to the food industry (as food coloring), cosmeticsm and pharmaceuticals (as an anti-oxidant and pro-vitamin A) has not been widely reported. Therefore, this study carried out for identifying and characterizating marine microalgae produced biopigment Thalassosira sp. In the study of marine microalgae Thalassiosira sp. cultivated for 6 days with a light intensity of the light:dark = 12:12. <br /> <br /> <br /> <br /> <br /> Harvested cells by centrifugation method; biopigmen extracted using acetone solvent; biopigment components separated by thin layer chromatography (TLC) using acetone <br /> <br /> <br /> <br /> <br /> eluent:hexane = 3:7; and each biopigment identified and characterized. Biomass concentration of Thalassiosira sp. obtained at 1.75 g/L. The result of TLC, TLC scanner, and <br /> <br /> <br /> <br /> <br /> spectrophotometer UV-Vis, showed that microalgae Thalassiosira sp. has differrent biopigment (at (lambda)max) include chlorophyll a(409 nm and 669 nm) and c(409 nm and 669 nm), carotenol (neoxanthin (410 nm, 439 nm, and 466 nm), violaxanthin (409 nm, 428 nm, and 453 <br /> <br /> <br /> <br /> <br /> nm), lutein(409 nm, 428 nm, and 452 nm), and fucoxanthin(447 nm and 469 nm)), carotene (beta-carotene (427 nm, 453 nm, and 473 nm)), and pheophythin (405 nm and 663 nm).