DEGRADATION OF USED TISSUE PAPERS USING RECOMBINANT ENDO-1,4-Βeta-GLUKANASE

• Main Author: RIZKI NOVIANDI (NIM : 10507070); Pembimbing : Zeily Nurachman, D.Sc, TRI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/17464
• Institution: Institut Teknologi Bandung

Used tissue papers are household waste containing high cellulose. Cellulose from such used tissue paper waste can be degraded to free sugar products that can be used for multiple purposes. In the present study, the degradation of used tissue paper with various brands using the recombinant endo-1,4-Beta-glucanase was investigated. Firstly, enzyme from recombinant Bacillus megaterium induced by xylosa was isolated. Secondly, used tissue papers were hydrolyzed by the recombinant endo-1,4-Beta-glucanase at three different variations of temperature. Finally, reducing sugar products were determined by the DNS method. The experimental results showed that used tissue paper waste (10 mg) from all various brands hydrolyzed by recombinant endo-1,4-Beta-glucanase at 50 oC for 5 days released 0.0007 mmol reducing sugar. Thus, it can be concluded that recombinant endo-1,4-Beta-glucanase can be used to hydrolyzed used tissue paper.