ISOLASI DAN IDENTIFIKASI SENYAWA AKTIF ANTI MIKROBA DARI DAUN TUMBUHAN Piper sarmentosum Roxb. Ex Hunter

• Main Author: Shinta
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/1877
• Institution: Institut Teknologi Bandung

Piper sarmentosum Roxb. ex Hunter is one of plant species from Piperaceae family which has been known as a traditional medicinal herb that cure many diseases. In Indonesia, the chemistry of this plant and its microbial activities have not been extensively investigated. This research was aimed to isolate the antimicrobial compounds of Piper sarmentosum leaves and tested its activity againts several different microorganisms. Dried leaves of Piper sarmentosum was methanol extracted by maseration, and then partitioned extracted with n-hexane, chloroform, and ethyl acetate. Further isolation and purification were performed by column chromatography, thin layer chromatography, and crystalisation. Identification of the pure compound isolated were carried out using ultra violet spectroscopy, infra red spectroscopy, and mass spectroscopy. The result indicated that from chloroform extract, a compound of esterification of phenylpropanoid derivative, with functional-group addition —CH3 and —OH on the benzena ring, could be purely isolated. This compound showed an antimicrobial activities, againts Candida alhicans, Escherichia coli, Bacillus subtilis, and Pseudomonas aeruginosa. At a concentration of 1250 .tg/mL, the observed inhibition growth area of Candida alhicans was 31 mm, whereas those for Escherichia colt and Bacillus subtilis were 23 mm. The optimum concentration againts Pseudomonas aeruginosa was 1000 µg/mL, which gives an inhibition growth area of 22 mm. It could be seen that the most effected and sensitive microorganism is Candida alhicans. This fact is line with the use of this plant by people tradition, in which the leaves of Piper sarmentosum was utilized as a medicinal herbs for treating fungoid diseases, especially at vagina and foot-sole.