#TITLE_ALTERNATIVE#

• Main Author: SEULINA PANJAITAN (NIM : 20511313); pembimbing : Prof. Fida Madayanti Warganegara, PhD, RIONG
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/19333
• Institution: Institut Teknologi Bandung

Indonesia is a maritime country that has a very diverse biological wealth. One of its wealth is brown seaweed (Sargassum polycistum) and green seaweed (Ulva fasciata) in Sayang Heulang Beach, Pameungpeuk, Garut, West Java. Because of the increasing resistance of bacteria and fungus, the discovery of new antibacterial and antifungal indispensable. Sargassum polycistum and Ulva fasciata extracted using chloroform and methanol with a volume ratio of 2:1 (Folch method) to obtain a crude extract lipids. Total lipid content of Ulva fasciata seaweed is 8.39% (from wet weight) and Sargassum polycistum 12.4% (from wet weight). Optimization of eluent for chloroform extract separation was obtained using thin layer chromatography which hexane and acetone with a composition of 7 to 3 was the optimized ratio. This eluent composition was applied for futher separation using column chromatography (silica G60 7731, column diameter is 1 cm). The results of the separation by column chromatography were characterized using visible spectrophotometry. Chloroform extracts of Sargassum polycistum contained betha carotene, alpha carotene, fucoxanthin and phaeophytin a while chloroform extracts of Ulva fasciata contained phaeophytin a, chlorophyll a and chlorophyll b. <br /> <br /> <br /> <br /> <br /> <br /> <br /> Crude extract lipids obtained from both types of seaweed were tested antibacterial activity against gram-positive bacteria (Bacillus cereus and Staphylococcus aureus) and gram-negative bacteria (Pseudomonas fluorescence, Pseudomonas aeruginosa, and Escherechia coli) by observing the clear zone formed around the paper disc . Crude extract of Sargassum polycistum lipid from chloroform fraction has vi antibacterial activity against E. coli of 3,5 mm/50 &#956;g extract while the methanol fraction has antibacterial activity against B. cereus, S. aureus and E. coli were 4 mm/50 &#956;g extract, 1 mm/50 &#956;g extract and 4 mm/50 &#956;g extract respectively. The crude extract lipid of Ulva fasciata from chloroform fraction has antibacterial activity against S. aureus and B. cereus were 10 mm/50 &#956;g extract and 8 mm/50 &#956;g extract while the methanol fraction has antibacterial activity against B. cereus, S. aureus and E. coli in a row of 4 mm/50 &#956;g extract, 9 mm/50 &#956;g extract and 8 mm/50 &#956;g extract. The methanol and chloroform was used as negative control, while the positive control was tetracycline. Average antibacterial activity formed on the crude extract of lipid from methanol fraction (Sargassum polycistum and Ulva fasciata) against E. coli is 13 mm/50 &#956;g extract while the negative control is 8 mm/50 &#956;g extract and 18 mm/50 &#956;g extract for the positive control. This suggests that the crude extract of lipid from methanol fraction (Sargassum polycistum and Ulva fasciata) have antibacterial activity against E. coli. Antibacterial and antifungal activity of pheophytin a fraction was tested. Pheophytin a showed its antibacterial to B.cereus and no antibacterial activity to S.aureus and E.coli. Then, the crude extract of Sargassum polycistum and Ulva fasciata lipid from chloroform fraction and pheophytin a have antifungal activity against Candida albicans.