KEMAMPUAN BAKTERI ASAM LAKTAT ( LACTOBACILLUS DELBRUEKII BEIJERINCK, L.FERMENTUM BEIJERINCK, L. PLANTARUM ORLA JENSEN) DALAM MENGHAMBAT PERTUMBUHAN DAN PRODUKSI AFLATOKSIN BL DARI ASPERGILLUS FLAVUS

• Main Author: TRI LUNGGANI, ARINA
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/2019
• Institution: Institut Teknologi Bandung

Aflatoxins are highly toxic secondary metabolies produced during the growth of several fungi, especially Aspergillus flavus. AFB, is one of them which contaminates a wide variety of food and feed causing serious health problem when consumed by human or animals. This research was aimed to study the ability of Lactic Acid Bacteria (LAB) in the inhibition of Aspergillus flavus growth and the production of Aflatoxin B1. Three species of LAB i.e. Lactobacillus delbrueckii , L. fermentum, L. plantarum were investigated for their abilities in inhibiting and degradation of Aflatoxin Bl as well as inhibiting fungal growth. The trial was designed into three variations of each isolate by challenging the fungal culture, before fungal inoculation, at the same time as fungal inoculation and after fungal inoculation. It was found that all the three species of LAB are potential microorganism to inhibit fungal growth as indicated by the reduction of the dry weight of fungal mycelia compared with control. Quantification of Aflatoxin Bl showed that L. fermentum gave the strongest degradation of Aflatoxin Bl during 15 days incubation, then followed by L. delbruekii and L. plantarum with a reduction rate 82,03% (0.1953 ppm), 71, 74% (0.308 ppm), 59,05% (0.4463 ppm) respectively, compared with control these are significantly different at confident interval 95 %. These result conclude that Aflatoxin B1 can be reduce or prevented to be produced by A. flavus by applying LAB.