#TITLE_ALTERNATIVE#

• Main Author: MUHAMMAD (10713058), ADAM
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/20702
• Institution: Institut Teknologi Bandung

Krangean (Litsea cubeba (Lour.) Pers.) is a plant from Lauraceae family and distributed abundantly throughout tropical and subtropical Asia, including Indonesia. Krangean has many benefits which used in traditional medication, e.g. toothache medicine and insect bites antitoxin. Previously, xanthine oxidase (XO) inhibition assay from krangean bark water extract was evaluated. This research was aimed toexamine XO inhibition activity of krangean bark methanol extract and isolate the marker compound. The study began with characterization and phytochemical screening of crude drugs. Krangean extract were obtained in methanol by reflux method, filtered, and concentrated. It was monitored by thin layer chromatography (TLC). The extract was also tested for XO inhibition activity. Fractionation was done by vacuum liquid chromatography and classic column chromatography. The subfractions were purified by preparative TLC and the purity was checked by single development TLC and two-dimensional TLC. The isolate was monitored by TLC with potassium hydroxide and Dragendorff spray reagents. The isolate showed blue fluorescence under λ 365 nm ultraviolet (UV) light and orange color under visible light. Furthermore, it was characterized with the UV-visible light spectrophotometer. It had maximum wavelengths at 228, 281, and 301 nm. Based on that characterization, the isolate was predicted to be coumarin compound. At 100μg/mL, the extract inhibited XO activity by 22,52±1,88%. The half maximal inhibition concentration (IC¬50) of the extract was 229,27 μg/mL.