#TITLE_ALTERNATIVE#

• Main Author: NUR KAMIL (NIM : 10511061), AHMAD
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/20854
• Institution: Institut Teknologi Bandung

Diatoms are microalgae-walled cell with a major component of the silica which has a complete amino acid content. Diatoms as feed for larval P.Indicus subphylum crustaceans provide 30 times survival better than the provision of nutritious pellets. The mean research is characterization of biosilica and determination of the protein content of the diatom Thalassiosira sp. as a first step to determine the amount of good nutrition in the breeding of larvae P.Indicus. The study begins with the cultivation of Thalassiosira sp., biomaass measurement and test of thermal stability, morphological characterization of functional groups biosilika, then extracted and determination of protein content in Thalassiosira sp. obtained wet biomass density Thalassiosira sp. after cultivation for 8 days was (3.1376 ± 0.06759) grams being (0.5213 ± 0.03213) grams after drying. Thermogravimetric analysis shows the dry biomass has a thermal stability at temperatures of 600-800 0C. Biosilicaa obtained for 12.08% of the dry biomass, with a composition of 94.2% SiO2 through EDS test. SEM characterization biosilica describe sheet-shaped morphology with large pores. FTIR test proves biosilica have functional groups Si-O-Si at a wavelength of 1099.43 cm-1 and has a water absorption at 3429.3 cm-1. Data SDS-PAGE showed the protein concentration Thalassiosira sp. using freeze-dried protein derived molecular weight is more varied than the concentration using a solvent TCA method. Results of the determination of protein content in Thalassiosira sp. with bradford assay amounted to 8.89% (w/w) while the test kjedahl is 9.02% (w/w).