MOLECULARLY IMPRINTED POLYMER AS SELECTIVE FUNCTIONAL MATERIAL FOR SEPARATION AND ANALYSIS OF RHODAMINE B

• Main Author: PINANDITA (NIM : 10513075), ARIA
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/21211
• Institution: Institut Teknologi Bandung

Rhodamine B was a synthetic dye of xanthene derivatives commonly used in the paint and textile industries. These dyes caused irritation of the respiratory system, has a carcinogenic effect, and at high concentrations caused liver damage. Rhodamine B has been used in processed foods because it provides a brighter and cheaper color. The aim of this research was to produce a molecularly imprinted polymer sorbents, (MIP), which could be used in more selective, accurate, and precise for rhodamine B analysis. Polymer synthesis were produced by bulk polymerization method using rhodamine B as template molecule, methacrylic acid and methyl methacrylate as functional monomer, ethylene glycol dimethacrylate as crosslinker, and benzoyl peroxide as initiator in acetonitrile as porogen solvent. With mol ratio of template molecule : functional monomer : crosslinkers of 1:4:20, resulted MIP with imprinting factor (IF) of 10,49 for MIP with methacrylic acid monomer and 7,76 for MIP with methyl methacrylate monomer. Both MIP types were characterized using an infra red spectrophotometry and evaluated for adsorption and desorption ability. Molecularly Imprinted Solid Phase Extraction (MISPE) with methacrylic acid monomer was more selective against erythrosin B. The analytical performance of separation method for rhodamine B analysis using MISPE showed linearity (R2), precision (RSD), detection limit, and quantitative limit were respectively 0,9982; 0,04%; 0,09 mg/L; and 0,28 mg/L. This MISPE were applied in analysis of red colored traditional cracker. Rhodamine B concentration in traditional cracker samples using MISPE and spectrofotometry UV-Vis were in a range between 35,03 μg/g to 85,57 μg/g.