STUDY OF BIOREMEDIATION OF CYANIDE DESTRUCTION BY PSEUDOMONAS PSEUDOALCALIGENES BACTERIA

• Main Author: PUTRO HAPSORO (NIM : 12509033), BIMO
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/21438
• Institution: Institut Teknologi Bandung

Cyanidation is used widely to extract gold from its ore in the world. But, there will always be a waste from every metallurgical process and cyanidation makes <br /> <br /> <br /> <br /> cyanide wastes which is hazardous for the environment. Thus, the waste must be treated properly, so the environment will not be contaminated (remediation) by <br /> <br /> <br /> <br /> destruction of cyanide into safer compound. The most widely methods used to destruct cyanide are INCO process and alkaline chlorination. Cyanide naturally occurs in the living organisms, it means that such organisms can utilize it. The most suitable organisms to be used to destruct cyanide are the bacteria because they have wide range of pH to survive. From those bacteria, Pseudomonas <br /> <br /> <br /> <br /> pseudoalcaligenes is chosen because it has proven to be able utilize cyanide as sole nitrogen source. <br /> <br /> <br /> <br /> In this experiment, bacteria from Sekolah Ilmu dan Teknologi Hayati - ITB was used as living organism to destruct cyanide. The bacteria were first cultured for 24 hours, and then they were inoculated in the cyanide solution with initial concentration 700 ppm. The first experiment was done by the variable of bacterial <br /> <br /> <br /> <br /> cultural solution volume fraction with number 1, 5, 10, and 20. After the best condition was obtained, the second experiment would be done by varying the concentration of acetate (25 mM, 50 mM, 75 mM, and 100 mM) and time of destruction process (1, 3, 5, and 7 days). As addition to economic side for the application, next experiment were done by varying bacterial cultural solution volume fraction by 0.1%, 0.5%, 1%, and 2%. <br /> <br /> <br /> <br /> First experiment resulted that 1% was the best cultural solution volume fraction, which was able to lower cyanide concentration from 689.47 ppm to 539.93 ppm. <br /> <br /> <br /> <br /> Second experiment resulted 25 mM and 7 days as the best concentrate of acetate and time of destruction process, respectively. And the last experiment resulted 0.1% of bacteria cultural solution volume fraction gave the best cyanide concentration depletion from 700 ppm to 590.42 ppm. <br /> <br /> <br /> <br /> This experiment reflects that cyanide destruction process by bacteria do not need large amount of bacteria as long as the bacteria get enough nutrients. For time of destruction process it is known that the longer it is, the more cyanide is depleted.