PENGARUH PEMBERIAN ELISITOR YANG BERASAL DARI JAMUR PYTHIUM APHANIDERMATUM (EDSON) FITZP. TERHADAP KANDUNGAN AJMALISIN DALAM KULTUR AKAR CATHARANTHUS ROSEUS (L) G. DON.

• Main Author: MUKARLINA
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/2166
• Institution: Institut Teknologi Bandung

An experiment to study the effect of elicitor from Pythium aphanidermatum (Edson) Fitzp. on ajmalicine content of Catharanthus roseus (L) G.Don. root culture has been conducted The Zenk media were used for root induction and root culture with addition of 10-' M Naphthalene Acetic Acid (NAA) and 10' Benzylaminopurine (BAP). The root culture was elicited with elicitor derived from autoclaved P. aphanidermatum at concentrations of 0.05, 0.5, 1.0 and 5.0 mg DW/ mL then harvested at 18, 24, 36, 48, and 72 hours after elicitation. The ajmalicine was analyzed qualitatively and quantitatively by using HPLC (High Pressure Liquid Chromatography) connected to chromatopack CR-7A plus. The result of elicitation showed that ajmalicine content was influenced significantly by concentration and harvesting time. The highest ajmalicine content on the roots (165,609 + 1,141 DW/g ) with the percentage of increment 181.633% was elicited by addition of 5.0 mg DW/mL elicitor, for 24 hours, while the highest ajmalicine content on the media (291,473 ± 0,872 mg DW/ L) with the percentage of increment (306, 466%) was elicited by addition of 5.0 mg DW/mL elicitor for 36 hours. This result indicated that elicitation might be responsible for further release of ajmalicine content into the media rather than its accumulation in the root.