BIOLOGI SPERMA PETAURUS BREVICEPS PAPUANUS T.

Several aspects of sperm biology of Petaurus breviceps papuanus T. have been observed, which include : sperm number, sperm morphology, sperm motility and sperm viability of in vitro as well as of cryopreserved sperms. Sperms were obtained mostly from the epididymis or the caudal part of _ epididymis...

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Main Author: MADE RAI SUARNI , NI
Format: Theses
Language:Indonesia
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Online Access:https://digilib.itb.ac.id/gdl/view/2175
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Institution: Institut Teknologi Bandung
Language: Indonesia
id id-itb.:2175
spelling id-itb.:21752004-10-25T16:32:23ZBIOLOGI SPERMA PETAURUS BREVICEPS PAPUANUS T. MADE RAI SUARNI , NI Ilmu hayati ; Biologi Indonesia Theses INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/2175 Several aspects of sperm biology of Petaurus breviceps papuanus T. have been observed, which include : sperm number, sperm morphology, sperm motility and sperm viability of in vitro as well as of cryopreserved sperms. Sperms were obtained mostly from the epididymis or the caudal part of _ epididymis except for the morphological data; sperm of the testis were also used. The results showed that the number of epididymal sperm are 5.37 million and 1. 67 million (31%) are located in the caudal part of epididymis. Sperm of the testis are T-shape, the acrosome is located on the medio-dorsal side of the head with a cytoplasmic droplet on the neck. Two shapes of the epididymal sperm were obtained, T-shape and spear-shape, the acrosome is button-shape and overlies the dorsal part of the sperm head, with one end reaching the anterior side of the sperm head, the neck is inserted into a groove located on the ventral side of the sperm head. Mitochondrial helix, which is characteristic for the midpiece, is unwrapped at the anterior part but the posterior part is wrapped with a plasma membrane invaginated with longitudinal grooves. The length of the sperm is 90.50 ± 0.71 µm. Two motility patterns could be observed, the spear-shape sperm move in a circle and the T-shape sperm move progressively by rotating the head. 4. 75% of the sperm could be maintained for 7.5 hrs in DMEM at 35°C. Sperm cryopreservation was still unsuccessful due to the unsuitable cryoprotectant in this experiment . text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
topic Ilmu hayati ; Biologi
spellingShingle Ilmu hayati ; Biologi
MADE RAI SUARNI , NI
BIOLOGI SPERMA PETAURUS BREVICEPS PAPUANUS T.
description Several aspects of sperm biology of Petaurus breviceps papuanus T. have been observed, which include : sperm number, sperm morphology, sperm motility and sperm viability of in vitro as well as of cryopreserved sperms. Sperms were obtained mostly from the epididymis or the caudal part of _ epididymis except for the morphological data; sperm of the testis were also used. The results showed that the number of epididymal sperm are 5.37 million and 1. 67 million (31%) are located in the caudal part of epididymis. Sperm of the testis are T-shape, the acrosome is located on the medio-dorsal side of the head with a cytoplasmic droplet on the neck. Two shapes of the epididymal sperm were obtained, T-shape and spear-shape, the acrosome is button-shape and overlies the dorsal part of the sperm head, with one end reaching the anterior side of the sperm head, the neck is inserted into a groove located on the ventral side of the sperm head. Mitochondrial helix, which is characteristic for the midpiece, is unwrapped at the anterior part but the posterior part is wrapped with a plasma membrane invaginated with longitudinal grooves. The length of the sperm is 90.50 ± 0.71 µm. Two motility patterns could be observed, the spear-shape sperm move in a circle and the T-shape sperm move progressively by rotating the head. 4. 75% of the sperm could be maintained for 7.5 hrs in DMEM at 35°C. Sperm cryopreservation was still unsuccessful due to the unsuitable cryoprotectant in this experiment .
format Theses
author MADE RAI SUARNI , NI
author_facet MADE RAI SUARNI , NI
author_sort MADE RAI SUARNI , NI
title BIOLOGI SPERMA PETAURUS BREVICEPS PAPUANUS T.
title_short BIOLOGI SPERMA PETAURUS BREVICEPS PAPUANUS T.
title_full BIOLOGI SPERMA PETAURUS BREVICEPS PAPUANUS T.
title_fullStr BIOLOGI SPERMA PETAURUS BREVICEPS PAPUANUS T.
title_full_unstemmed BIOLOGI SPERMA PETAURUS BREVICEPS PAPUANUS T.
title_sort biologi sperma petaurus breviceps papuanus t.
url https://digilib.itb.ac.id/gdl/view/2175
_version_ 1820663178441261056