#TITLE_ALTERNATIVE#

• Main Author: Dista Maisa Pusporini , Fonna
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/22197
• Institution: Institut Teknologi Bandung

Organohalogen is a compound that is commonly used to produce plastics, PVC, medicines, pestisides, and herbicides. Organohalogen is classified as xenobiotic, and persistent in the environment for a long time. Excessive accumulation of organohalogen in human body can cause liver dysfunction, kidneys dysfunction, nerve system malfunction, and even congenital defects. One of most used organohalogen is monochloroacetic acid (MCA). Researchs of monochloroacetic acid degrading bacteria was done to support MCA bioremediation. Some local strain bacteria was screened for its activity to degrade MCA, that is Pseudomonas aeruginosa 1, Pseudomonas aeruginosa 2, Pseudomonas fluorescens, Azotobacter chroococcum, Bacillus cereus, dan Staphylococcus aureus using MCA as the substrate in Minimal Medium (MM). These bacteria were able to grow in LB medium that contains 0,5 mM MCA, which indicates that these bacteria can tolerate the presence of MCA. MCA degradation test was conducted with Bergmann and Sanik method to quantify the concentration of Cl⁻ released to the medium. In MM that does not contain glucose, Pseudomonas aeruginosa 1 was able to degrade 2,04% of 18 mM MCA, Pseudomonas aeruginosa 2 was able to degrade 3,59% of 12 mM MCA, and Pseudomonas fluorescens was able to degrade 0,48% of 15 mM MCA, while Azotobacter chroococcum, Bacillus cereus, and Staphylococcus aureus was not able to degrade MCA. In MM that contains 0,005% (w/v) glucose, Azotobacter chroococcum was able to degrade 2,86 % of 8 mM MCA and Bacillus cereus was able to degrade 9,25% of 5 mM MCA. In the similar MM, Staphylococcus aureus needed 0,01% (w/v) glucose to degrade 4,43% of 5 mM MCA. Ribotyping identification was conducted to confirm the species of the highest MCA degrading bacteria, Pseudomonas aeruginosa 1 and Pseudomonas aeruginosa 2 is Pseudomonas aeruginosa, while Pseudomonas fluorescens is Limnobacter sp.