ELECTROSPUN GELATIN/CELLULOSE ACETATE FIBERS AS A CARRIER OF MANGOSTEEN PERICARP EXTRACT

• Main Author: HERNAWATI KUSUMAH (NIM: 20214023, FUJI
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/22222
• Institution: Institut Teknologi Bandung

Mangosteen pericarp extract contains xanthone which could potentially be used as a therapeutic drug. However, it is difficult to dissolve in water and poorly absorbed when consumed. Nanofiber is a medium that could increase the solubility of a compound. In this study, the method used for produce nanofiber was electrospinning. The focus of this research was to make gelatin/cellulose acetate nanofibers as a drug carrier of mangosteen pericarp extract. From SEM images, it was shown that the average diameters of gelatin/cellulose acetate fibers from the precursor solutions of 10:0, 8:2, and 6:4 are 534, 748, and 835 nm, respectively. The addition of cellulose acetate increased the viscosity of gelatin/cellulose acetate solution. Moreover, gelatin mass reduction caused the decrease in conductivity of gelatin/cellulose acetate solution. Therefore, increasing in the viscosity or reducing in the conductivity of the precursor solution increased the average diameter of the gelatin/cellulose acetate fibers. The analysis of FTIR spectra showed that the structural changes of gelatin and cellulose acetate occurred after being transformed into gelatin/cellulose acetate nanofibers. Besides, the gelatin/cellulose acetate/mangosteen pericarp extract fibers at mass ratios of 6:1, 8:1 and 12:1 had average diameters of 359, 525, and 682 nm, respectively, in which there were beads on the fibers for a mass ratio of 6:1. The XRD pattern showed that electrospinning process causes mangosteen pericarp extract structure change from crystalline to amorphous structure. The addition of mangosteen pericarp extract caused the decrease in viscosity and the increase in conductivity of gelatin/cellulose acetate solution. Meanwhile, gelatin/cellulose acetate/mangosteen pericarp extract 8:1 with flow rate variations of 0.1, 0.2, and 0.3 mL/h had average diameters of 525, 640, and 698 nm, respectively. In vitro release study showed that the release pattern of mangosteen pericarp extract from gelatin/cellulose acetate/mangosteen pericarp extract fibers was composed of two phases, comprising rapid release and gradual release. The mangosteen pericarp extract release rate was affected by the size of the fiber, in which the smaller the fibers average diameter, the faster the release rate of mangosteen pericarp extract from the fibers.