#TITLE_ALTERNATIVE#

• Main Author: GANJAR PRABOWO ( NIM : 15310081 ), HIMAWAN
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/22455
• Institution: Institut Teknologi Bandung

One of the cases that often among in Indonesia which associated with oil and gas industries is soil contaminated oil. The methode to handling this is with biology process using the bacteria. Before doing the process, bacteria must have tested to know that ability for petroleum degradation. The purpose of this research is to find the best isolate for degrading petroleum compound. This reasearch is used local bacteria that obtained from soil contaminated oil. The research begin with isolation bacteria from soil contaminated oil to rich nutrition agar. Bacteria were grown selected then purified and tested in SBS (Standard Basal Salt) liquid medium with the addition of various types of oil to be tested. The oil will be tested are kerosene, diesel, and oil. Each oil is tested to be varied with the composition of 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, and 0.6%. Oil degradation test performed for 7 days with 1 day of observation time span once. The results were found two kind of isolates that have skill to degradation oil that isolates are isolate A-1 (Bacillus simplex) and isolate A-2 (Bacillus firmus). Bacteria degaradation test of kerosene, diesel, and oil did to know kinetic growth bacteria. Result from kinetic growth were show that Bacillus simplex had Ks = 4,26 g/L and μmaks = 0,090/day on kerosene, Ks = 3,68 g/L and μmaks = 1,240/day on diesel, and Ks = 2,06 g/L and μmaks = 0,240/day on oil and for Bacillus firmus Ks = 0,69 g/L and μmaks = 0,056/day on kerosene, Ks = 8,00 g/L and μmaks = 1,127/day on diesel, and Ks = 13,36 g/L and μmaks = 0,543/day on oil. From that result know Bacillus simplex is the best for petroleum degradation especially for diesel.