THE ELECTROSPINNING OF GARCINIA MANGOSTANA L. LOADED-POLYVINYLPYRROLIDONE (PVP) NANOFIBERS AS A CARRIER MEDIUM TO IMPROVE THE BIOAVAILABILITY OF α-MANGOSTIN

• Main Author: SRIYANTI (NIM: 30214001), IDA
• Format: Dissertations
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/22486
• Institution: Institut Teknologi Bandung

The compound of &#945;-mangostin in a mangosteen pericarp can potentially be used as a therapeutic drug. However its use is limited by its low bioavailability. The synthesis of nanofibers as the drug carrier system is one way to increase the bioavailability &#945;-mangostin. The nanofiber can be produced through single needle as well as needleless electrospinning. The nanofibers of polyvinylpyrrolidone (PVP) with encapsulated Garcinia Mangostana Extract (GME) was successfully synthesized while the needleless electrospinning set have been successfully developed. For single needle electrospinning, the most optimum fibers was FG3 (SSJ) with average diameter of 387 nm with parameters used were a voltage of 12 kV, flow rate of 0.5 mL/hr and needle-collector distance of 12 cm. <br /> <br /> <br /> The development of needleless electrospinning apparatus using straight wire collector yield FG3 (STJ) with average diameter of 1,02 &#956;m as the most optimum fibers. The process parameters used for the needleless electrospinning were the voltage of 40 kV and wire-collector distance of 14 cm. The production rate of fibers by the needleless electrospinning was higher than the single needle electrospinning system. XRD and DSC results showed that the electrospinning caused the crystal structure of &#945;-mangostin to become amorphous. The results of Fourier Transform Infra Red (FTIR) spectroscopy for the nanofiber produced through single needle electropinning indicated that the addition of GME did not change the molecular structure of PVP, whereas the fibers FG3 (STJ) generated from the needleless electrospinning showed a change of PVP molecular structure with the appearance of a new peak at 1578 cm-1 which was the stretch of C=C aromatic groups. The results from the in vitro study showed that the release of &#945;-mangostin from fiber FG3 (SSJ) and FG3 (STJ) were faster than GME. Results of stability tests confirmed that the fiber FG3 (STJ) was more stable than the fiber FG3 (SSJ) in protecting the &#945;-mangostin when placed at room temperature. When the in vivo tests using winstar rats was performed, the fiber FG3 (STJ) was able to enhance the bioavailability of &#945;-mangostin compared to pure GME.