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Identification of microalgae species is a crucial stage for classifying microalgae. The identity of microalgae species is very important for the purposes of collection and conservation so that microalgae can be utilized in a sustainable manner. So far, a conventional morphological identification of...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/22594 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Identification of microalgae species is a crucial stage for classifying microalgae. The identity of microalgae species is very important for the purposes of collection and conservation so that microalgae can be utilized in a sustainable manner. So far, a conventional morphological identification of microalgae is less accurate for some microalgal lineages because of similarities between species and cell simplicity. Therefore, additional data in the form of genetic information is required to identify microalgae species faster and more accurate. This study aimed to investigate the two speciments of the tropical marine microalgae named as NLO and NPL through a cell morphology and phylogenetic analysis using 18S rDNA and LSU D2/D3 marker genes. Cell morphology of microalgae was potraited under light microscope and by SEM (Scanning Electron Microscope). The results showed that speciment NLO had a round shape with 3–4 μm in diameter, green, and irregular cell surface. Based on the nucleotide sequences of 18S rDNA, speciment NLO had 94% similarity with Micractinium sp. Further identification using LSU D2/D3 gene marker, speciment NLO had 96% similarity with Micractinium reisseri. Based on cell morphology characteristics and nucleotide sequence similarities the speciment NLO belonged to the genus Micractinium. Meanwhile, microalgae NPL had an oval in shape with a diameter of 22,5–25 μm, brown, and transparent shell. Speciment NPL showed of typical characteristics of the morphology of Navicula. This was consistent with the similarity of nucleotide sequence of 18S rDNA area V4 of Navicula cf. lundii to 99% as well as the similarity of nucleotide sequence of LSU D2/D3 gene of Navicula mollis to 97%. Based on cell morphology characteristics and nucleotide sequence similarities the speciment NPL belonged to the genus Navicula. |
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