TRANSFORMATION OF BFE (BACTERIAL FAD EXPORTER) GENE ON Escherichia coli BL21 AS ELECTROGENS IN MICROBIAL FUEL CELL (MFC) SYSTEM

• Main Author: Widata (NIM 10612009), Jimmy
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/22699
• Institution: Institut Teknologi Bandung

Microbial Fuel Cell is a renewable technology using microbes as energy source. Microbes often used in MFC system is Shewanella oneidensis MR-1 because of its ability to transfers electrons on solid metal dan flexibility on anaerobic environment. One known method that can help this electron transfer is by using electron shuttle mechanism in form of flavins and MtrCAB complex. Secretion of flavins is done by multidrug and toxin efflux transporter then later named as bfe (bacterial flavinadenine dinucleotide [FAD] exporter) (Acc: NC_004347.2). This research purpose is to determine electric potential of Escherichia coli that is transformed by bfe gene. bfe gene used is syntetic gene inserted to pET-23a(+) vector by GenScript Company. E.coli made competent transformed by bfe gene then SDS-PAGE method is used to confirm the expression after IPTG induction. Transformed and non-transformed E.coli then tested on MFC system. MFC used is dual chamber MFC. Electric parameter is measured by Arduino Uno as multimeter and data is collected by PLX-DAQ and Arduino software. Highest OCV for non-transformed E.coli is 0,51V with average 0,482V and highest OCV for transformed E.coli is 0,55V with average 0,516V. Peak power density for non-transformed E.coli is 201,38 mW/m2 when peak current density is 958,54 mA/m2 and resistance is about 300Ω, Peak power density for transformed E.coli is 240,75 mW/m2 when peak current density is 1046,76mA/m2 and resistance is about 400Ω